Denaturation and Renaturation of Xanthosine 5′-Phosphate Aminase
نویسندگان
چکیده
منابع مشابه
Formation of an adenyl xanthosine monophosphate intermediate by xanthosine 5'-phosphate aminase and its inhibition by psicofuranine.
The amination of xanthosine 5’-phosphate and its inhibition by psicofuranine was examined with substrate amounts of purified xanthosine St-phosphate aminase from Escherichia coli. In the absence of NH,, incubation of the enzyme with adenosine triphosphate-8-i4C and XMP-8J4C for 10 min results in the conversion of ATP to AMP without concomitant formation of guanosine monophosphate. Shorter perio...
متن کاملEnzymes essential for the biosynthesis of nucleic acid guanine; xanthosine 5'-phosphate aminase of Aerobacter aerogenes.
Earlier studies have shown1 X5’P to be an obligatory intermediate in the biosynthesis of nucleic acid guanine in Aerobacter aerogenes and related organisms (24). The present paper describes the isolation of X5’P aminase, the enzyme responsible for the conversion of X5’P to G5’P, from extracts of A. aerogenes, and discusses the mechanism of this reaction and its role in purine metabolism. A simi...
متن کاملInduction of Inosine F-Phosphate Dehydrogenase and Xanthosine S-Phosphate Aminase by Ribosyl-4-
Examination of various purine-requiring mutants of Escherichia coli strain B revealed that one strain, B-35-2, blocked in the conversion of 5-amino-1-(5’-phosphoribosyl)-4-imidazole carboxamide to inosine 5’-phosphate, possessed markedly elevated levels of inosine 5’-phosphate dehydrogenase and xanthosine 5’-phosphate aminase when grown under conditions of purine limitation (1). In contrast, an...
متن کاملBiosynthesis of guanosine 5'-phosphate. I. Xanthosine 5'-phosphate as an intermediate.
The work of Greenberg (1) and Buchanan et al. (2) has established inosine 5’-phosphate as a key intermediate in the biosynthesis of hypoxanthine in pigeon liver. The recent indication of the enzymic synthesis of adenosine 5’-phosphate from IMP1 (3-5) shows a possible role of IMP as an intermediate in the biosynthesis of other purine nucleotides. In order to investigate the possibility of the en...
متن کاملCharacterization of denaturation and renaturation of DNA for DNA hybridization
OBJECTIVES The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was de...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44841-2